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Organizations face ongoing threats to their information technology infrastructure daily. These security struggles need to be approached with modern techniques and a diverse body of knowledge. With the proper tools and training, specialists in the Cybersecurity fields will be more capable of finding success in their roles. The Introduction to Cybersecurity Defense and Operations textbook addresses the critical topics and skills needed to ensure a robust security posture is maintained. Readers will be presented with knowledge of core foundational principles such as Threat Intelligence Analysis, Vulnerability Management, Biometric Systems, Incident Response, Securing Systems with Cryptography, and implementation strategies of the NICE Cybersecurity Workforce Framework. The target audience for this textbook includes Cybersecurity Analysts, college students pursuing Information Assurance and Technology disciplines, and entry-level professionals employed in any cybersecurity role.